Antagonistic Effects of Two Trichoderma Species on Pathogen of Rice Seedling Blight in Cold Region

doi:10.3969/j.issn.1006-8082.2020.04.022

Abstract

Abstract: The antagonistic activities of Trichoderma harzianum and Trichoderma brevis against four pathogens causing rice seedling blight in cold regions were analyzed by confrontation culture, fermentation broth, non-volatile and volatile inhibitors. The results showed that both Trichoderma harzianum and Trichoderma brevis inhibited four pathogens to some extent. In confrontation culture, Trichoderma harzianum inhibited Fusarium oxysporum with the highest inhibition rate of 79.04% and Trichoderma brevis inhibited Fusarium graminearum with the highest inhibition rate of 88.65%. The non-volatile substances of Trichoderma spp. had strong inhibitory effects on four pathogenic bacteria, among which Fusarium graminearum had the strongest inhibitory effects, with inhibition rates of 66.78% and 70.12%, respectively. The chitinase activity of Trichoderma harzianum was higher than that of Trichoderma brevis. The results will lay a foundation for the research and development of Trichoderma complex fungus preparation to control rice seedling blight.

Key words: Trichoderma, metabolic substances, inhibitory effects, volatile substances, chitinase

摘要： 为了明确哈茨木霉和短密木霉对水稻立枯病菌的拮抗作用，采用对峙培养、发酵液、非挥发性和挥发性抑制物抑菌活性检测，分析了哈茨木霉和短密木霉对引起寒地水稻立枯病4种病原菌的拮抗活性。结果表明，2种木霉菌对4种病原菌均有一定的抑制作用。对峙培养，哈茨木霉对尖孢镰孢菌的抑制率最高为79.04%，短密木霉对禾谷镰孢菌的抑制率最高为88.65%。2种木霉非挥发性物质对4种病原菌抑制作用均较强，其中，对禾谷镰孢菌抑制作用最强，抑制率分别为66.78%和70.12%。通过几丁质酶活性测定，发现哈茨木霉比短密木霉产生更高的几丁质酶活性。本研究结果可为研发木霉复合菌制剂防治水稻立枯病奠定基础。

关键词: 木霉, 代谢物质, 抑制作用, 挥发性物质, 几丁质酶

CLC Number:

S435.111.4+6

战鑫1,台莲梅1*,刘铜2*,张有利1,郑雯1,靳亚忠1. 2种木霉菌对寒地水稻立枯病病原菌的拮抗作用研究[J]. 中国稻米, DOI: 10.3969/j.issn.1006-8082.2020.04.022 .

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